Laminated diagnosis and treatment sheets for Lyme Disease and Spotted Fever, designed by members of the TDRU, are also available for doctors by contacting:
Jan Gray (02 9976 9531) at the Health Promotion Unit, Manly Hospital, PO Box 465, Manly NSW 2095.Perform diagnostic procedures for Australian Spotted Fever if:
| Blood sample | Timing | Container | Volume |
|---|---|---|---|
| First | Day of presentation for illness | Citrate (INR) Clotted blood |
5 mL 10 mL |
| Second | 14-21 after onset of illness | Citrate (INR) Clotted blood |
5 mL 10 mL |
Store all serum and culture samples at cool room temperature, out of direct light.
Send as soon as possible after collection, through usual pathology provider, to:
Dr Bernie Hudson, Microbiology Dept, Royal North Shore Hospital, St Leonards, 2065.If have suspicious signs/symptoms and diagnostic blood samples have been collected
Print version in doctor's algorithms (pdf, 2MB).

Perform diagnostics for Lyme-like disease if:
If appropriate, please send a skin biopsy for Lyme culture, before giving antibiotics, to:
PaLMS at Royal North Shore Hospital.For specific instructions about skin biopsy collection and transportation to PaLMS call 02 9926 8480.
| Sample type | Container | Volume |
|---|---|---|
| Blood sample (initial presentation for illness) | Citrate (INR) Clotted blood |
5 mL 10 mL |
| Skin Biopsy: 4 mm punch biopsy from margin of lesion - divided into 2 portions |
Formalin saline in std container Normal saline in sterile tube |
- 5-10 mL |
| Skin Aspirate | Sterile tube | 5 mL |
| Cerebrospinal Fluid | Sterile tube | 5-10 mL |
| Synovial Fluid | Sterile tube Citrrate (INR) |
5-10 mL 5 mL |
Prompt treatment of early suspected Lyme-like disease may prevent chronic debilitating infection.
Initial treatment:
Pregnant women and children:
Print version in doctor's algorithms (pdf, 2MB).
